Rat Nephrin ELISA, Ethos

The Rat Nephrin ELISA kit contains all of the components and reagents you need to assay rat nephrin in up to 40 duplicate urine or cell free liquid samples (plasma, serum, cell culture supernatant, etc…). This assay has been carefully developed to remove cross reactivity to albumin. Nephrin is a transmembrane protein expressed in renal glomerular podocytes; studies in experimental animals and human diabetes suggest nephrinuria is a marker for glomerular filtration dysfunction or podocyte injury. As an indirect competitive ELISA, this kit uses an HRP labeled antibody to detect minute levels of nephrin, primarily in urine. This kit contains 1 x 96 well plate, and allows for 80 samples or 40 samples in duplicate (recommended) to be analyzed per kit. Overnight incubation allows users to prepare the assay on the first day and assess the data on the second day.

Kit Contents include:

• One Rat Nephrin Assay Plate
  
• Two EIA Diluent
  
• One Rat Nephrin Standard
  
• One Anti-rat Nephrin Antibody
  
• One Anti-mouse IgG-HRP Conjugate
  
• One Color Developer/Stopper
  
• Instructions