{"product_id":"tbars-malondialdehyde-oxidative-stress-assay","title":"TBARS MDA Oxidative Stress Assay","description":"\u003cdiv class=\"ewa-rteLine\"\u003eThe Ethos Biosciences TBARS Malondialdehyde Oxidative Stress assay measures thiobarbituric acid reactive substances in biological specimens. Reactive oxygen species are a consequence of oxidative metabolism, which are balanced by reducing capacity under normal conditions. Under disease conditions, this balance may be lost causing oxidative stress damage such as lipid peroxidation. Upon metabolic breakdown of altered lipids, formation of “secondary messengers” including malondialdehyde (MDA), can occur allowing measurement of MDA as an assessment of oxidative stress.\u003c\/div\u003e\n\u003cdiv class=\"ewa-rteLine\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"ewa-rteLine\"\u003eThe TBARS Malondialdehyde Oxidative Stress Assay depends on the reaction of thiobarbituric acid and MDA under acidic conditions to form an adduct. Results are reported in MDA equivalents. This assay comes with all the reagents needed to run 80 samples or 40 in duplicate (recommended) using either fluorometric or spectrophotometric methods on a microplate.\u003cem\u003e\u003c\/em\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ewa-rteLine\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-family: var(--global-body-font-family);\"\u003eKit Contents:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eTBA Stock Solution\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eMDA Standard: 300 m\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eTBARS Assay Diluent: 10X PBS must be diluted with water before use\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eTBARS Reaction Buffer: 50% Acetic Acid. 2.5 mL of TBA stock is added to this buffer to make TBA working reagent.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eMicrotiter plate that may be used with a plate reader in either fluorometric or in a spectrophotometric modes.\u003cbr\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cstrong\u003eMaterials required, but not supplied in the kit:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eReaction tubes: for 500 l reaction volumes, 1.7 mL microfuge tubes work well. For 2.5 mL reaction volumes, 16 x 120 mm screw-cap glass tubes work well.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eWater bath or heat block set at 75\u003csup\u003eo\u003c\/sup\u003e C\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eAdjustable pipets\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eDisposable pipets\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eGraduated cylinder\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003ePurified water\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eDisposable gloves\u003c\/li\u003e\n\u003cli\u003eAssay detection device:\n\u003col\u003e\n\u003cli\u003ePlate reader: fluorometry: ex: 532 nm\/em 553 nm\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003ePlate Reader Spectrophotometry: OD 532 nm\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eFluorometer\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eUV-Vis Spectrophotometer\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Ethos Biosciences","offers":[{"title":"Default Title","offer_id":52440608145690,"sku":"1020","price":363.74,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1002\/7558\/5306\/files\/wp-image11692712951908-scaled.png?v=1784918134","url":"https:\/\/shop.ethosbiosciences.com\/products\/tbars-malondialdehyde-oxidative-stress-assay","provider":"Ethos Biosciences","version":"1.0","type":"link"}