TBARS MDA Oxidative Stress Assay
$363.74
Sale price
$363.74
Regular price
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TBARS MDA Oxidative Stress Assay
$363.74
Sale price
$363.74
Regular price
The Ethos Biosciences TBARS Malondialdehyde Oxidative Stress assay measures thiobarbituric acid reactive substances in biological specimens. Reactive oxygen species are a consequence of oxidative metabolism, which are balanced by reducing capacity under normal conditions. Under disease conditions, this balance may be lost causing oxidative stress damage such as lipid peroxidation. Upon metabolic breakdown of altered lipids, formation of “secondary messengers” including malondialdehyde (MDA), can occur allowing measurement of MDA as an assessment of oxidative stress.
The TBARS Malondialdehyde Oxidative Stress Assay depends on the reaction of thiobarbituric acid and MDA under acidic conditions to form an adduct. Results are reported in MDA equivalents. This assay comes with all the reagents needed to run 80 samples or 40 in duplicate (recommended) using either fluorometric or spectrophotometric methods on a microplate.
Kit Contents:
- TBA Stock Solution
- MDA Standard: 300 m
- TBARS Assay Diluent: 10X PBS must be diluted with water before use
- TBARS Reaction Buffer: 50% Acetic Acid. 2.5 mL of TBA stock is added to this buffer to make TBA working reagent.
- Microtiter plate that may be used with a plate reader in either fluorometric or in a spectrophotometric modes.
Materials required, but not supplied in the kit:
- Reaction tubes: for 500 l reaction volumes, 1.7 mL microfuge tubes work well. For 2.5 mL reaction volumes, 16 x 120 mm screw-cap glass tubes work well.
- Water bath or heat block set at 75o C
- Adjustable pipets
- Disposable pipets
- Graduated cylinder
- Purified water
- Disposable gloves
- Assay detection device:
- Plate reader: fluorometry: ex: 532 nm/em 553 nm
- Plate Reader Spectrophotometry: OD 532 nm
- Fluorometer
- UV-Vis Spectrophotometer
- Plate reader: fluorometry: ex: 532 nm/em 553 nm
Weight
2.0 lb