Drabkin's Microplate Hemoglobin Assay, 1 kit, Exocell
Drabkin's Microplate Hemoglobin Assay, 1 kit, Exocell
Drabkin’s Microplate Hemoglobin Assay is for the determination of hemoglobin in blood samples, column fractions, and any sample with visible hemoglobin. It comes with 1x96 well plate allowing for 80 samples, or 40 in duplicate (recommended). The Drabkin’s Microplate Hemoglobin Assay converts the variable hemoglobin forms in blood samples to cyanmethemoglobin which can be read spectrophotometrically at 540 nm. Compared to the standard cuvette-based assay, the microplate assay uses less sample and is amenable to high-throughput. The dose response of the assay is linear from 0.5 mg/ml to 20 mg/ml. Hemoglobin can exist in multiple forms depending on oxygen content, carbon monoxide content, and oxidative state of the iron (ferrous, ferric, and ferryl). The heterogeneous nature of hemoglobin can be overcome by conversion to cyanmethemoglobin. The resulting cyanmethemoglobin has a peak absorbance at 540 nm and an extinction coefficient of 0.68 for a 1 mg/ml solution. RUO not for IVD.
Kit Contents:
- 1 x 96-well Assay Plate
- 1 x Hemoglobin Standard
- 2 x Drabkin's Reagent
Other Materials required but not provided:
- Microplate reader equipped to measure absorbance at 540 nm is required
- Adjustable pipettes and pipette tips
- Multi-channel pipettor(s) and tips for dispensing 150 µl volumes
- Tubes for preparing dilutions, i.e. microfuge tubes
